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The P app was calculated using the following formula: where P app is the permeability coefficient, t is the time of incubation in seconds, V HD is defined by the volume of medium in the hanging-drop compartment, C HD is the measured concentration of FITC-dextran and LY in the hanging-drop compartment at incubation time t , V MC is the volume of the microchannel, C MC is the measured concentration of FITC-dextran and LY in the microchannel at time t , and A is the permeable area of the microchannel in cm 2
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