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in terms of quantity, there is a “threshold effect”: a study of Indian middle school students (28) indicated that when daily protein intake was ≥1.2 g/kg body weight, intestinal tryptophan supply was stable, 5-HT (5-hydroxytryptamine) synthesis was sufficient, and depression scores decreased significantly (mean difference = −1.8 points, P 1 g/day) has a small effect on moderate depression, while low-dose supplements (≤ 0.5 g/day) provide no benefit—this dose-response relationship was not discussed in the original analysis (65, 66)