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Purity was then normalized between time points by setting purity at time 0 ( T = 0) to 100 for each pH value for a given peptide, as follows: Assessment of the Chemical Stability of Peptide 40 and Aggregation of Peptide 40 and Semaglutide Testing of Peptide 40 in Formulations at Neutral pH Peptide 40 was dissolved in basic Milli-Q water (adjusted to pH = 9 with NaOH) to concentrations ranging from 10 to 30 mg/mL, and the resulting solutions were diluted to achieve a peptide 40 concentration of 1, 4, or 10 mg/mL